Amgen Jobshttp://www.amgen.jobs2012-05-26 15:16:32.873076CANNoneAuto req 15574BR Job Posting Title Co-op Student: Hybridoma Subcloning and ESN Generation Career Category College Job Employee Subgroup Co-Op FT Salaried Country (State/Region) Canada Location (City) Burnaby Amgen Job Description Amgen British Columbia Inc., one of several research facilities operated by Amgen Inc., specializes in the discovery and development of human therapeutic antibodies. The research center, located in Burnaby, became part of Amgen with Amgen’s acquisition of Abgenix, Inc. in April 2006. It employs approximately 65 people, including research scientists and a small support staff. Amgen is headquartered in the United States and has more than 17,000 staff members worldwide. A biotechnology pioneer since 1980, Amgen was one of the first companies to realize the new science’s promise by bringing novel medicines from lab, to manufacturing plant, to patient. Amgen therapeutics have changed the practice of medicine, helping millions of people around the world in the fight against cancer, kidney disease, rheumatoid arthritis, and other serious illnesses. With a deep and broad pipeline of potential new medicines, Amgen remains committed to advancing science to dramatically improve people’s lives. This project involves the discovery of novel human therapeutics or diagnostics using XenoMax™ and Hybridoma technology. These two technologies allow identification of single immune cells from a large population that produces very high affinity antigen specific monoclonal antibodies. Another key component of the company is the Xenomouse™, which is a proprietary transgenic mouse capable of producing fully human antibodies. The marriage of these technologies allows us to produce human antibodies against targets that would normally be considered self”. The candidate will be working in the Cell Biology/Subcloning group, which is responsible for using a FACs ARIA single cell sorter to isolate hybridoma’s of interest. Hybridoma’s are then scaled up and used to generate ESN supernatant, which will contain antibody of interest for further assays. The candidate will be responsible for reagent generation, maintenance of both FACS Caliburs and Aria, use of FACsAria for single cell sorting, cell culture, cell banking and harvesting of supernatants. The candidate will also be involved with our Robotics liquid handling platforms to aid in the above work. Anticipated Achievements: • Learn and implement basic cell culture techniques such as sterile technique, splitting cells, optimizing growth conditions, cryovial preservation of mammalian cells, media preparation for various cell lines • Gain experience in both FACS Calibur and Aria systems as well as Robotics • Gain much valuable experience in time organization and multitasking. • Become proficient in background searches and internet research. • Independently carry out projects depending on needs of company All candidates will be subject to the verification of current college enrollment as well as a Canadian criminal records check prior to employment. Amgen retains the right to add or change the duties of the position at any time. As an EEO/AA M/F/V/D employer, Amgen values and encourages diversity in the workplace Basic Qualifications • Must be in the process of earning a Bachelors of Science degree in Chemistry, Molecular Biology, Biochemistry, Biology/Microbiology, Life Sciences, Chemical Engineering, or related discipline. • Must be able to work for two consecutive four month terms with continued employment for the second term dependant on a positive performance review at the end of the first term. • Must be NSERC eligible . Amgen requires that all individuals applying for an internship or a co-op assignment at Amgen must meet the following criteria: • Must be 18 years or older • Must be a current full time student • Must have completed at least one full year of study • Must have not yet graduated • Must be in good academic standing within their majors and overall at their university • Must be enrolled in school the fall season following the potential internship or co-op assignment • Must not be employed at the time the internship starts Preferred Qualifications • Prefer students with previous lab experience. • Prior experience in tissue culture is desired, experience in immunology and immunofluorescence techniques would be preferred. • General knowledge of immunology & molecular biology would be an asset. • Excellent organizational skills and attention to detail. • Function effectively as part of a team.2012-05-03 18:02:03CanadaAmgenNone15574BRNoneVirtual, CAN28403685http://www.amgen.jobs/xml/28403685/jobCANNoneAuto req 15576BR Job Posting Title Co-op Student: Cell Based Screening & Assay Development Career Category College Job Employee Subgroup Co-Op FT Salaried Country (State/Region) Canada Location (City) Burnaby Amgen Job Description Amgen British Columbia, one of Burnaby’s premier biotechnology companies, is focused on the development and commercialization of therapeutic antibody products targeting disease prevention and treatment. The company's antibody technology platform enables the rapid generation and selection of high affinity, fully human antibody product candidates to essentially any disease target appropriate for antibody therapy. Working in partnership with our U.S. based parent company, Amgen Inc., Amgen British Columbia leverages its leading technology, flexible manufacturing capabilities and a network of partnerships for the discovery, development and manufacture of therapeutic antibodies for serious health conditions. For more information, please visit our website at www.amgen.com. Job Summary: The student will be part of a high-energy, fast paced team responsible for cell screening and assay development in our antibody generation process. The team utilizes Flow Cytometry and FMAT as the screening platforms and also utilizes high capacity liquid handling robotics. In addition to becoming proficient in our screening platforms and process, the student is expected to maintain a variety of cell lines and is responsible for cell transfections. The student will have the opportunity to develop novel assays to assist in characterizing lead antibodies. Essential Functions: • Performs a variety of Cell-based assays: • assays used to characterize the immune response in immunized animals • high-throughput cell screens to identify antigen specific antibodies • functional assays to characterize antibodies for epitope specificity, ligand blocking and species reactivity • Gain strong working knowledge of Flow Cytometry, FMAT and ELISA • Critically evaluate data and present results to the team • Tissue culture and transfections • Optimize/develop assays All candidates will be subject to the verification of current college enrollment as well as a Canadian criminal records check prior to employment. Amgen retains the right to add or change the duties of the position at any time. As an EEO/AA M/F/V/D employer, Amgen values and encourages diversity in the workplace Basic Qualifications • Must be in the process of earning a Bachelors of Science degree in Chemistry, Molecular Biology, Biochemistry, Biology/Microbiology, Life Sciences, Chemical Engineering, or related discipline. • Must be able to work for two consecutive four month terms with continued employment for the second term dependant on a positive performance review at the end of the first term. • Must be NSERC eligible. Amgen requires that all individuals applying for an internship or a co-op assignment at Amgen must meet the following criteria: • Must be 18 years or older • Must be a current full time student • Must have completed at least one full year of study • Must have not yet graduated • Must be in good academic standing within their majors and overall at their university • Must be enrolled in school the fall season following the potential internship or co-op assignment • Must not be employed at the time the internship starts Preferred Qualifications • Prefer students with previous lab experience. • General knowledge of immunology & molecular biology would be an asset. • Excellent organizational skills and attention to detail. • Function effectively as part of a team.2012-05-03 18:01:15CanadaAmgenNone15576BRNoneVirtual, CAN28403677http://www.amgen.jobs/xml/28403677/jobCANNoneAuto req 15575BR Job Posting Title Co-op Student: In Vivo Career Category College Job Employee Subgroup Co-Op FT Salaried Country (State/Region) Canada Location (City) Burnaby Amgen Job Description Amgen British Columbia, one of several research facilities operated by Amgen Inc., specializes in the discovery and development of human therapeutic antibodies. The research center, located in Burnaby, became part of Amgen with Amgen’s acquisition of Abgenix, Inc. in April 2006. It employs approximately 50 people, including research scientists and a small support staff. Amgen is headquartered in the United States and has more than 17,000 staff members worldwide. A biotechnology pioneer since 1980, Amgen was one of the first companies to realize the new science’s promise by bringing novel medicines from lab, to manufacturing plant, to patient. Amgen therapeutics has changed the practice of medicine, helping millions of people around the world in the fight against cancer, kidney disease, rheumatoid arthritis, and other serious illnesses. With a deep and broad pipeline of potential new medicines, Amgen remains committed to advancing science to dramatically improve people’s lives. Job Summary The successful candidate will work as part of the Preclinical group at Amgen British Columbia Inc. The student will be responsible for performing a variety of in vivo and in vitro cell biology techniques used to evaluate potential cancer targets and oncology drug candidates. Experiments will be conducted in a semi-independent fashion under supervision. There are a variety of projects currently ongoing in the Preclinical group and these projects change frequently. The student will be involved in some or all of these projects as needed. Essential Functions The job involves assisting researchers in all aspects of in vivo and in vitro studies. There will be some routine aseptic cell culture and flow cytometry. In addition, depending on current projects student may learn some or all of the following: In vivo studies: study design, data collection, treatment formulation prep, animal handling, tumor measurement and data analysis. In Vitro studies: ELISA, SDS-PAGE, Western Blots and cell-based assays evaluating cytokine release, T cell or NK cell activation, proliferation or apoptosis. All candidates will be subject to the verification of current college enrollment as well as a Canadian criminal records check prior to employment. We request that all applicants include a cover letter with their resume when they apply. Applicants without a cover letter may not be considered for the position. Amgen retains the right to add or change the duties of the position at any time. As an EEO/AA M/F/V/D employer, Amgen values and encourages diversity in the workplace Basic Qualifications • Must be in the process of earning a Bachelors of Science degree in Chemistry, Molecular Biology, Biochemistry, Biology/Microbiology, Life Sciences, Chemical Engineering, or related discipline. • Must be able to work for two consecutive four month terms with continued employment for the second term dependant on a positive performance review at the end of the first term. • Must be NSERC eligible . Amgen requires that all individuals applying for an internship or a co-op assignment at Amgen must meet the following criteria: • Must be 18 years or older • Must be a current full time student • Must have completed at least one full year of study • Must have not yet graduated • Must be in good academic standing within their majors and overall at their university • Must be enrolled in school the fall season following the potential internship or co-op assignment • Must not be employed at the time the internship starts Preferred Qualifications • Suitable for a first or second work term as all training will be provided. • General knowledge of In Vivo studies, immunology & molecular biology would be an asset. • High attention to detail and able to comply with written procedures. • Strong written and oral communications skills in English. • Excellent organizational skills. • Function effectively as part of a team.2012-05-03 18:01:04CanadaAmgenNone15575BRNoneVirtual, CAN28403671http://www.amgen.jobs/xml/28403671/job